Part:BBa_K759002:Design

E. coli agregation (Ag43) device induced by T7 promoter.

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 758
  Illegal NgoMIV site found at 1982
  Illegal NgoMIV site found at 2492
  Illegal NgoMIV site found at 2513
  Illegal AgeI site found at 1508
  Illegal AgeI site found at 2252
  Illegal AgeI site found at 2666
  Illegal AgeI site found at 2908
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

We found at that part (BBa_K542009) is lacking Lac1 promoter and RBS.So we need to construct it with basic parts. And add terminators.

Source

Peking 2010 team parts.